Streptomyces albus G Mutants Defective in the SalGI Restriction-Modification System
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چکیده
منابع مشابه
Restriction of a bacteriophage of Streptomyces albus G involving endonuclease SalI.
The bacteriophage Pa16, isolated from soil on Streptomyces albus G, was restricted when transferred from an alternative host back to S. albus G. Extracted unmodified Pa16 deoxyribonucleic acid was cleaved at a single site by a cell-free extract of S. albus G. Fractions cleaving Pal6 deoxyribonucleic acid contained the endonuclease SalI first described by J. Arrand, P. Myers, and R. J. Roberts (...
متن کاملThe exoceliular , B - lactamase of Streptomyces albus G
The exoceilular f-lactamase of Streptomyces albus G has been purified to near protein homogeneity. It consists of one single polypeptide chain of mol.wt. 30000-31000, has a rather low isoelectric point (at pH 6.0) and contains less lysine (2.1%) and more half-cystine residues than most fi-lactamases from other Gram-positive bacteria. Penicillins are much better substrates than A3-cephalosporins...
متن کاملThe active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.
The active-site serine of the extracellular beta-lactamases of Streptomyces cacaoi and Streptomyces albus G has been labelled with beta-iodopenicillanate. The determination of the sequence of the labelled peptides obtained after trypsin digestion of the denatured proteins indicate both enzymes to be class A beta-lactamases. Surprisingly the two Streptomyces enzymes do not appear to be especiall...
متن کاملThe crystal structure of the beta-lactamase of Streptomyces albus G at 0.3 nm resolution.
The crystal structure of the beta-lactamase of Streptomyces albus G has been solved at 0.3 nm resolution by X-ray-diffraction methods. The enzyme is a typical two-domain protein. One domain consists of five alpha-helices, and the other is five-stranded beta-sheet with alpha-helices on both sides of the sheet. The active-site serine residue (Ser-48) is within a cleft located between the two doma...
متن کاملCloning the BamHI restriction modification system.
BamHI, a Type II restriction modification system from Bacillus amyloliquefaciensH recognizes the sequence GGATCC. The methylase and endonuclease genes have been cloned into E. coli in separate steps; the clone is able to restrict unmodified phage. Although within the clone the methylase and endonuclease genes are present on the same pACYC184 vector, the system can be maintained in E. coli only ...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1980
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-116-2-323